What is Sepsc?
Southeastern Program Service Center (SEPSC)
What is an mepsp?
Miniature postsynaptic currents are currents observed in the absence of presynaptic action potentials; they are thought to correspond to the response that is elicited by a single vesicle of transmitter.” (1)
How do I record mEPSC?
Hold the voltage at -60 mV to record the mEPSC events for 5 min. Change the holding potential to 0 mV to record mIPSC events for 5 min. Because M1 neurons show slightly higher reversal potential for AMPA receptor-mediated currents, the mIPSCs of M1 neurons are recorded at +15 mV with 0.1 mM APV.
What affects mEPSC frequency?
Two factors that could produce the increased mEPSC frequency noted above are increases in the number of docked vesicles per synapse and/or an increase in the number of synapses. We have examined both of these possibilities.
What is a miniature EPSC?
Miniature EPSC (mEPSC) analysis indicates pre- rather than post-synaptic effects of LY354740. A: Original current traces of mEPSC recorded in an individual CeLC neuron in the presence of TTX (1 μM) show that LY354740 (1 μM) reduces frequency but not amplitude of mEPSCs.
What are EPSPs and IPSPs?
EPSP is a temporary depolarization that is caused by the flow of positively-charged ions into the postsynaptic cell while IPSP is a hyperpolarization caused by the flow of negatively-charged ions into the postsynaptic cell.
What are EPSPs and IPSPs and how are they produced?
At many other synapses, PSPs actually decrease the probability that the postsynaptic cell will generate an action potential. PSPs are called excitatory (or EPSPs) if they increase the likelihood of a postsynaptic action potential occurring, and inhibitory (or IPSPs) if they decrease this likelihood.
What factors could explain an apparent decrease in mEPSC amplitude?
Any of the factors you mentioned could change mEPSC amplitude, namely, less neurotransmitter content per quanta (per presynaptic vesicle), fewer postsynaptic receptors, or a change in the type of receptors at postsynaptic sites (i.e., a change in the single channel conductance of ligand gated receptors).
What is miniature EPSC?
What is the Sepsc frequency?
The frequency of sEPSCs was also quite variable, ranging from 0.2 to 5 Hz. sEPSCs were equivalent to miniature EPSCs recorded in the presence of tetrodotoxin, and both sEPSCs and emEPSCs were CNQX-sensitive.
What causes mini EPSPs?
EPSPs in living cells are caused chemically. When an active presynaptic cell releases neurotransmitters into the synapse, some of them bind to receptors on the postsynaptic cell. This depolarizing current causes an increase in membrane potential, the EPSP.
How are IPSPs generated?
An inhibitory postsynaptic potentials (IPSP) is a temporary hyperpolarization of postsynaptic membrane caused by the flow of negatively charged ions into the postsynaptic cell. An IPSP is received when an inhibitory presynaptic cell, connected to the dendrite, fires an action potential.