What is RetroNectin?
RetroNectin is a recombinant human fibronectin (CH-296) that consists of three functional domains: the human fibronectin cell-binding domain (C-domain), heparin-binding domain (H-domain), and CS-1 sequence domain.
How do you dilute a RetroNectin?
1. Add the retrovirus stock solution or diluted solution at 125 – 500 μl/cm2 to the RetroNectin-coated plate. Note: The volume that can be added to the plate varies. For a 6-well plate, the upper limit is 3 ml (320 μl/cm2).
Does RetroNectin work for lentivirus?
RetroNectin reagent can be used to enhance transduction of VSV-G, ecotropic, and amphotropic retroviruses and lentiviruses. In addition, this product is compatible with retroviruses with a GALV-type envelope.
What is LentiBOOST?
LentiBOOST® is a highly effective, non-cytotoxic transduction enhancer for preclinical and clinical application of lentiviral vectors. It is a universal polaxamer-based, receptor-independent adjuvant which facilitates fusion of lenitivrus particles with cell membrane, significantly increasing transduction efficiency.
Can RetroNectin be reused?
4) How often can reconstituted RetroNectin® be frozen and reused? Reconstituted RetroNectin® (1 mg/ml) can be used without problems with repeated freeze-thaw cycles up to 5 times. However, avoid excess freeze-thaws and be careful to prevent contamination.
What is retronectin®?
What is RetroNectin®? RetroNectin ® , recombinant human fibronectin fragment, is a protein of ~63 kDa (574 amino acids) containing a central cell-binding domain (type III repeat, 8, 9, 10), a high affinity heparin-binding domain II (type III repeat, 12, 13, 14), and CS1 site within the alternatively spliced III CS region of human fibronectin.
Can retronectin reagent be used to stimulate CD8+ T cells?
Peripheral blood T cells were stimulated and expanded in vitro using anti-CD3 (clone OKT3) and IL-2 alone or in combination with RetroNectin reagent (RN), anti-CD28, or anti-4-1BB. Stimulation with RetroNectin reagent yielded higher proportions of cytotoxic CD8+ T cells. Pack of ten 35 mm dishes that are pre-coated with RetronNectin reagent.
How does retronectin enhance gene transduction?
RetroNectin enhances lentiviral- and retroviral-mediated gene transduction by aiding the colocalization of target cells and viral particles.
Can I use retronectin reagent for high-efficiency viral transduction?
A 300-ml format (which can be coated with RetroNectin reagent) is available for high-efficiency viral transduction of hematopoietic cells and a 1,000-ml format can be used to expand cell cultures following transduction. View our protocol and see how to set up your cell culture in a single small bag and then expand it into multiple larger bags.