What is PNA FISH?


What is PNA FISH?

Fluorescence in situ hybridization using peptide nucleic acid probes (PNA FISH) is a novel diagnostic technique combining the simplicity of traditional staining procedures with the unique performance of PNA probes to provide rapid and accurate diagnosis of infectious diseases; a feature that makes PNA FISH well suited …

What is PNA FISH test?

PNA-FISH is a U.S. Food and Drug Administration (FDA) approved commercially available method for the detection of bacteria and yeast species directly from positive blood culture bottles. This methodology utilizes hybridization of PNA probes to organism-specific rRNA, with detection via fluorescent microscopy [6,7].

What is the hybridization buffer used in the lab?

DNA Hybridization Buffer is a modified Denhardt’s solution in citrate buffer containing formamide and a mixture of blocking reagents. When used as a diluent for DNA targeting probes in an ISH procedure, DNA Hybridization Buffer facilitates spreading of probes and reduces drying of DNA during hybridization reactions.

What is PNA DNA?

Peptide nucleic acid (PNA) is an artificially synthesized polymer similar to DNA or RNA. Synthetic peptide nucleic acid oligomers have been used in recent years in molecular biology procedures, diagnostic assays, and antisense therapies.

What is PNA molecule?

Abstract. Peptide nucleic acids (PNAs) are synthetic mimics of DNA in which the deoxyribose phosphate backbone is replaced by a pseudo-peptide polymer to which the nucleobases are linked.

What is SSC used for?

Saline Sodium Citrate (SSC) is used as a hybridization buffer or a wash buffer in Southern blotting, in situ hybridization, DNA microarrays, or Northern blotting.

What is solution hybridization?

solution hybridization is designed to measure the levels of a specific mRNA species in a complex population of RNA. An excess of radioactive probe is allowed to hybridize to the RNA, then single-strand specific nuclease is used to destroy the remaining unhybridized probe and RNA.

What is PNA biology?

PNA (Peptide Nucleic Acid) is an artificially synthesized polymer similar to DNA or RNA. The various purine and pyrimidine bases are linked to the backbone by methylene carbonyl bonds as in peptides.