What are incompatibility groups?
Incompatibility grouping represents “the inability of two plasmids to coexist stably over a number of generations in the same bacterial cell line” such that plasmids incompatible to each other are assigned the same group [5].
What is replicon typing?
Since plasmid replicon type determines Inc group, the terms Inc and Rep type to describe plasmid types are used interchangeably. Initially, Inc typing relied on introduction of a plasmid into a strain carrying another plasmid and determining whether both plasmids were stably maintained in the progeny.
What determines copy number of plasmid?
Plasmids vary widely in copy number depending on three main factors: 1) The ori and its constituents – (e.g. ColE1 RNA I and RNA II). 2) The size of the plasmid and its associated insert (bigger inserts and plasmids may be replicated at a lower number as they represent a great metabolic burden for the cell).
What are the different types of plasmid?
There are five main types of plasmids: fertility F-plasmids, resistance plasmids, virulence plasmids, degradative plasmids, and Col plasmids.
What is a non conjugative plasmid?
Plasmids that cannot be transferred between bacteria through a pilus are called nonconjugative plasmids. The nonconjugative plasmid ColE1 is relatively low in molecular mass and does not encode the necessary gene required for it to be transferred from one cell to another.
What are plasmid replicons?
The replicon is comprised of the origin of replication (ori) and all of its control elements. The ori is the place where DNA replication begins, enabling a plasmid to reproduce itself as it must to survive within cells.
What is the difference between replicon and plasmid?
is that plasmid is (cytology) a loop of double-stranded dna that is separate from and replicates independently of the chromosomes, most commonly found in bacteria]], but also in archaeans and [[eukaryote|eukaryotic cells, and used in genetic engineering as a vector for gene transfer while replicon is (genetics) a dna …
What is a donor strain?
A donor strain, carrying a mobilizable plasmid that can utilize the transfer functions of the conjugative plasmid. A recipient strain, you wish to introduce the mobilizable plasmid into.
What is plasmid compatibility?
Plasmid incompatibility refers to the inability of two plasmids to coexist stably over a number of generations in the same bacterial cell line. Generally, closely related plasmids tend to be incompatible, while distantly related plasmids tend to be compatible.
What is high copy and low copy plasmid?
A way to determine experimentally if the copy number of your plasmid is high or low is to perform a miniprep. A high-copy plasmid should yield between 3-5 ug DNA per 1 ml LB culture, while a low-copy plasmid will yield between 0.2-1 ug DNA per ml of LB culture.